It’s ok, they have an ebola vaccine now, fortified with added “trust in strangers” chemicals. You won’t remember a thing.
The present invention is a method for enhancing the immune response of a mammal to a vaccine by administering to such a mammal an effective amount of GM-CSF in conjunction with a vaccine. The present invention further provides for pharmaceutical compositions containing an effective amount of GM-CSF and a vaccine. Kits containing GM-CSF and a vaccine are also disclosed….
To obtain a stronger humoral and/or cellular response, it is common to administer a vaccine in a formulation containing an adjuvant. An adjuvant is a substance that enhances, nonspecifically, the immune response to an antigen, or which causes an individual to respond to an antigen who would otherwise without the adjuvant not respond to the antigen. An adjuvant is usually administered with an antigen, but may also be given before or after antigen administration. Suitable adjuvants for the vaccination of mammals include but are not limited to Adjuvant 65 (containing peanut oil, mannide monooleate and aluminum monostearate); Freund’s complete or incomplete adjuvant; mineral gels such as aluminum hydroxide, aluminum phosphate and alum; surfactants such as hexadecylamine, octadecylamine, lysolecithin, dimethyldioctadecyl-ammonium bromide, N,N-dioctadecyl-N’,N’-bis(2-hydroxymethyl) propanediamine, methoxyhexadecylglycerol and pluronic polyols; polyanions such as pyran, dextran sulfate, poly IC, polyacrylic acid and carbopol; peptides such as muramyl dipeptide, dimethylglycine and tuftsin; and oil emulsions. The antigens could also be administered following incorporation into liposomes or other microcarriers….
According to the present invention, we have surprisingly found that the immune response in a mammal, especially a human, to a vaccine can be effectively enhanced by the administration of an effective amount of GM-CSF in conjunction with the vaccine. The term “in conjunction with” as used herein refers to the administration of GM-CSF concurrently, before or following administration of a vaccine.
As used herein, “GM-CSF” means a protein which (a) has an amino acid sequence that is substantially identical to the sequence of mature (i.e., lacking a signal peptide) human GM-CSF described by Lee et al. Proc. Natl. Acad. Sci. U.S.A. 82: 4360 (1985) and (b) has biological activity that is common to native GM-CSF….
To prepare the sustained release preparation of GM-CSF according to Formulation 2, the aluminum monostearate is mixed into the peanut oil for the gel and heat elevated to form the gel according to known methods.
The dioctyl sodium sulfosuccinate is dissolved into the Water for Injection. The lyophilized GM-CSF is reconstituted with the dioctyl sodium sulfosuccinate solution, the resultant solution is transfered into the peanut oil for emulsion and mixed by vortexing. The resultant emulsion is then mixed into the previously prepared gelled peanut oil and mixed by vortexing….
To prepare the sustained release preparation of GM-CSF according to Formulation 3, the monobasic and dibasic sodium phosphates are dissolved in a portion of the Water for Injection. The lyophilized GM-CSF is then dissolved in this solution and the pH is adjusted to 7.8 with the sodium hydroxide. The copper acetate is then added and the solution is agitated. The solution is brought to final volume using the remaining Water for Injection. Preferably, the sodium hydroxide and copper acetate are added as concentrated aqueous solutions (e.g., for copper acetate, 100 microliters of a 2 mg/ml aqueous solution).
Additional Sustained Release Formulations
Additional sustained release formulations of GM-CSF can be prepared using micoencapsulated or microspheres of GM-CSF prepared using polymers such as polyanhydrides, polyphosphazenes, collagen, alginates, poly(methacrylates), gelatin, poly(hydroxybutyrate), poly(caprolactone), ethylene vinyl acetate or polylactide glycolide….
Well sure why not? I mean it’s all FDA approved right? Why not add some FDA approved laundry detergent and EPA approved industrial smokestack scrapings with a side of hexadeathamine to wash it all down? And for good measure, inject it directly into the brain with a 5 gram “carrier” of Pb.
Their use of “substantially identical” gene sequences to GM-CSF is also comforting. I mean, it’s mostly junk dna anyway, right? Or it will be in short order.
Thank goodness for merck that this has nothing to do with the mysterious and sudden epidemic of deadly peanut allergies in children.
US Secretary for State Mike Pompeo has come under fire after a recording emerged of him saying he’d intervene to stop Labour Party leader Jeremy Corbyn becoming Prime Minister. In the recording first reported by the Washington Post, Pompeo suggests he won’t wait for Corbyn to be elected, rather he’ll attempt to stop it from being possible.
The off the record meeting was from when The Secretary for State met Jewish leaders to discuss Donald Trump’s proposed Peace Deal between Palestine and Israel.
Apart from appearing un-optimistic about the Peace Deal, he found time to comment on UK politics. When asked by an attendee;
if Corbyn “is elected, would you be willing to work with us to take on actions if life becomes very difficult for Jews in the U.K.?”
Pompeo gives a very clear response. Considering the allegations of Russian interference in the 2016 Presidential elections, the irony here is inescapable. The top US diplomat told those in attendance;
“It could be that Mr. Corbyn manages to run the gantlet and get elected. It’s possible. You should know, we won’t wait for him to do those things to begin to push back. We will do our level best,
“It’s too risky and too important and too hard once it’s already happened.”
This response received a voracious round of applause from those in the meeting.
These remarks raise serious questions about US interference in UK Politics. It has been alleged by many, that Russia played a part in the United Kingdom’s decision to leave the European Union (EU). Now a top US official wants to influence a General Election or perhaps have Corbyn removed as Labour Party leader…..
New Zealand politicians who rushed to enact nationwide gun confiscation following the Christchurch mosque massacres are befuddled by the lack of enthusiasm from citizens who have yet to comply with the new law. The so-called “gun reform” was expected to rid the vast New Zealand countryside of most semi-automatic firearms, magazines over a specified limit, and shotguns.
Two months ago, Reuters breathlessly reported, “New Zealand police expect tens of thousands of firearms to be surrendered by a guns buy-back scheme.” Law enforcement authorities averred that “it could be more.” Pregnant with the expectation that gun owners would trade their firearms for cash, the political class is nonplussed by the results.
Only 530 guns have thus far been turned in to the authorities.
Out of My Cold, Dead Hands
Figures released by the New Zealand police had politicians and law enforcement officials scrambling to comprehend what just happened. Michael Clement, the police deputy commissioner, assessed the situation by telling the media that the number of guns expected to be handed over is “a great unknown question,” primarily because the firearms the government is confiscating have never been registered with authorities….